Regulatory

Part:BBa_K1968009

Designed by: Alejandra Adriana Schiavon Osorio   Group: iGEM16_Edinburgh_OG   (2016-10-12)


PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

The Aspergillus a-glucoamylase (GlaA) enzyme is involved in starch utilization. The glaA promoter is induced by starch, maltose, and low concentrations of glucose but repressed by xylose (Fowler, Berka, & Ward, 1990). Basal transcription of glaA requires only 214 bp upstream of the start codon, while a high-level transcription expression requires the upstream region including -562 and -318 bp in the promoter region. The glaA promoters among different Aspergillus species are conserved.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 611
    Illegal SpeI site found at 280
    Illegal PstI site found at 147
    Illegal PstI site found at 489
    Illegal PstI site found at 605
    Illegal NgoMIV site found at 525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 579


[edit]
Categories
Parameters
None